Fig. 4.

G6PD-controlled cytokine secretion achieved by STAT1 signaling. A The StarBase 3.0 website examined the gene expression of STAT1, STAT3, and RELA in healthy persons (n = 291) and breast cancer patients (n = 1085). B Immunoblot analysis of the proteins in MDA-231-shG6PD, MCF10A-pcG6PD and corresponding control cells. C MDA-231 cells were treated with 6-AN, parthenolide or fludarabine. The levels of proteins were assessed by immunoblotting. D MDA-231 cells were harvested and subjected to immunoprecipitation with anti-G6PD, followed by Western blot analysis with the indicated antibodies. E Western blot analysis was used to detect indicated proteins from the cytosolic and nuclear extracts. F Schematic diagram showing the potential binding sites in the promoter region for STAT1. G, H q-PCR was performed to determine gene abundance of CCL2 or TGF-β1 promoter region in the groups of positive control (Input), negative control (IgG) and IP, which were immunoprecipitated with anti- phospho-STAT1 antibody in MDA-231 cells (G) and statistically analyzed (H). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001