Fig. 3.

PML-bodies formation. It is shown schematically that at the first stage, a liquid–liquid phase separation should occur, leading to a manifold increase in the concentration of scaffold proteins due to weak interactions, only after this the formation of specific interactions such as S–S bonds, RBCC motive, etc., which ultimately lead to the formation of mature MLO, in the case of PML-bodies they are large toroidal bodies are possible. Disordered regions of scaffold proteins are primarily involved in the formation of nonspecific interactions. In the case of PML proteins, these are the C-termini of PML-isoforms. Designations used in the figure are given in the lower left corner. Schematic presentation of different alternatively spliced isoforms of human PML protein are also shown: Mean disorder score determined by averaging the outputs of, IUPred2A Short IUPred2A Long, PONDR^® VLXT, PONDR^® VL3, PONDR^® VSL2B and PONDR^® FIT. The curve is superimposed with the positions of cysteine residues (black circles in N-terminal and red circles in C-terminal) and lysine residues (green circles); The generalized domain structure, indicating positions of the Ring domain (R—amino-acid residues 45–105), Box 1 (B1—residues 124–166), Box 2 (B2—residues 184–230), Coil-coil (CC—residues 229–323), Positions of the nuclear localization signal (NLS—residues 476–490), SUMO-interacting motif (SIM—residues 556–562) and nuclear export signal (NES—residues ~ 704–713, exclusive to isoform I); PML-I exons; The length of each isoform (colored line) and the length of the fragment in which the amino acid residues are the same in PML-I isoforms (black line)