Fig. 1.

Upregulation of Sec62 in GC predicts poor survival. Volcano plots of mass spectrometry data showed the top 10 upregulated molecules identified in GC tissues versus controls (A). Sec62 mRNA expression was significantly higher in GC than in other human malignancies (B) and adjacent normal stomach tissues (C, D) in the GEPIA database. Correlation of Sec62 mRNA expression in GC patients with OS in KM-plotter database (E). Quantitative PCR (F) and WB (G) assays suggested that Sec62 expression in GC cell lines was much higher than that in GES. In GC tissues and adjacent normal tissues, qRT-PCR (H) and WB (I) assays also confirmed higher Sec62 levels in GC tissues than in normal controls. IHC assays of Sec62 protein expression in GC and adjacent normal tissue (J). Chi-squared test for Sec62 expression in GC and adjacent normal tissues (K). Kaplan–Meier analysis using tissue microarray data showed a correlation between Sec62 protein levels and the OS of GC patients (L). **p < 0.01, ***p < 0.0001