Fig. 2.

Upregulation of Sec62 promotes GC cells migration and invasion in vitro and in vivo. After lentivirus transfection, WB assays verified the protein expression of Sec62 in three GC cell lines, namely, AGS (A), MKN45 (B) and GES (C). Transwell assays showed that Sec62 significantly promoted cell migration and invasion in AGS (D), MKN45 (E), and GES (F) cell lines. Scratch healing assays confirmed that upregulation of Sec62 enhanced GC cell migration in GC cell lines (G–I). Representative images of lung tissues from nude mice (J). Hematoxylin and eosin staining of the lungs and liver (K). The incidence of lung metastasis (L) and the number of metastatic nodules in the lungs (M) between the two groups**p < 0.01, ***p < 0.0001