Table 4.
The correlation between cytokine-producing CD4+ and CD8+ T cells with severity of neurological disabilities and depressive symptoms
| Cytokine expression (%) | αCD3/CD28 | MBP/IL-2 | ||||||
|---|---|---|---|---|---|---|---|---|
| EDSS | BDI | EDSS | BDI | |||||
| r | p | r | p | r | p | r | p | |
| CD4+ T cells | ||||||||
| IL-17+ | − 0.1832 | 0.4781 | − 0.027 | 0.9189 | 0.3415 | 0.2321 | − 0.207 | 0.4745 |
| IFN-γ+ | 0.1979 | 0.4588 | 0.2209 | 0.4081 | 0.1246 | 0.6424 | − 0.4321 | 0.0647 |
| GM-CSF+ | − 0.1457 | 0.6322 | − 0.0219 | 0.9493 | − 0.1862 | 0.5239 | 0.0285 | 0.9276 |
| IL-22+ | − 0.045 | 0.8734 | − 0.1737 | 0.5331 | − 0.3825 | 0.1297 | − 0.4295 | 0.1262 |
| CD8+ T cells | ||||||||
| IL-17+ | − 0.2119 | 0.4109 | 0.1314 | 0.6130 | 0.597 | 0.0211 | − 0.0088 | 0.9750 |
| IFN-γ+ | 0.0329 | 0.9042 | 0.0427 | 0.8757 | 0.4875 | 0.0293 | − 0.2474 | 0.3529 |
| GM-CSF+ | − 0.1075 | 0.7266 | − 0.2795 | 0.3551 | 0.2369 | 0.4112 | − 0.1385 | 0.6375 |
| IL-22+ | − 0.136 | 0.6290 | − 0.188 | 0.4994 | − 0.3337 | 0.2437 | − 0.3987 | 0.1580 |
Bold values are statistically significant (p < 0.05)
The frequency of CD4+ and CD8+ T cells positives for IL-17, IFN-γ, GM-CSF and IL-22 was determined by flow cytometry after PBMC cell cultures activation with anti-CD3/anti-CD28 beads (αCD3/CD28) or MBP/IL-2
EDSS expanded disability status scale, BDI beck depression inventory (BDI)