Fig. 6.

Inhibiting necroptosis alleviated FD-induced liver hypertrophy. a Larvae exposed to apoptosis inhibitor Q-VD-Oph (Q, 50 µM) or necroptosis inhibitor NSA (5 µM) starting from 7 dpf were imaged and quantified for liver size at 11 dpf. b The hepatic cryo-sections prepared from the larvae at 11 dpf were subjected to TUNEL assay for apoptotic cells. No apparent apoptotic signal was found in the liver (circled) of both control and FD larvae, as compared to positive control (sample pretreated with DNase). Statistical data are shown in mean ± SEM. CTL control (Tg-GGH/LR larvae without FD), FD folate deficiency