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. Author manuscript; available in PMC: 2024 May 6.
Published in final edited form as: Science. 2023 Nov 10;382(6671):eadf0966. doi: 10.1126/science.adf0966

Fig. 4: Deletion of intestinal Asters protects from diet-induced hypercholesterolemia.

Fig. 4:

(A) Body weight of WT (n = 13) and B/C KO (n = 11) male mice after 21 days of a western diet + 1.25% cholesterol (HC). (B) Plasma cholesterol levels after 4 h fasting in mice described in A. (C) Liver cholesterol in mice described in A. (D) Plasma cholesterol levels after 4 h fasting in F/F (n = 17) and I-B/C KO (n = 11) male mice after 21 days of HC diet. (E) Body weight of mice described in D. (F) FPLC of plasma from F/F and I-B/C KO mice fed for 21 days with HC diet and euthanized after overnight fasting followed by 2 h of refeeding with the HC diet (pool of 3–5/group). (G) Gene expression in distal jejunum scrapings from WT (n = 12) and B/C KO (n = 11) mice after 21 days of HC diet, euthanized after 4 h fasting. (H) Gene expression in distal jejunum scrapings from F/F (n = 6) and I-B/C KO (n = 5) male mice after 21 days of HC diet, euthanized after 4 h fasting. (I) Western blot analysis of duodenum scrapings of mice described in H (n = 4/5). (J) Lipidomic analysis of CE in proximal jejunum scrapings from mice described in H (n = 5/group). Data are expressed as mean ± SEM. Statistical analysis: unpaired t test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.