Fig. 2.

CHIP suppresses cell proliferation, invasion, and glycolysis in vitro. A, B Immunoblot analysis revealed overexpression and knockdown levels of CHIP in 11Z cells. Western blot was quantified by ImageJ software, and statistics were normalized to β-actin. C, D Effect of CHIP overexpression or knockdown on the proliferation of 11Z cells. E Overexpression of CHIP in 11Z cells resulted in a significant decrease in the number of colony formation. Knockdown of CHIP in 11Z cells resulted in a significant increase in the number of colony formation. F Overexpression of CHIP in 11Z cells resulted in diminished cell invasion. Knockdown of CHIP in 11Z cells resulted in enhanced cell invasion. G CHIP overexpression suppressed endometriotic cells migration. CHIP knockdown enhanced endometriotic cells migration. H, I The effects on glucose consumption and lactate production after overexpression or knockdown of CHIP are indicated, respectively. (All data represent mean ± SEM. The Student’s t test was used for data analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)