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. 2022 Dec 19;80(1):13. doi: 10.1007/s00018-022-04637-z

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© The Author(s), under exclusive licence to Springer Nature Switzerland AG 2022, corrected publication 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 4 — CHIP interacts with HMGB1. Perform immunoprecipitation and immunoblot analysis with specified antibodies. A, B Transfected Flag-tagged CHIP and HA-tagged HMGB1 into HEK293T cells and verified their interaction by immunoprecipitation and immunoblotting. C, D Immunoprecipitation and immunoblotting to verify the interaction of endogenous CHIP and HMGB1 proteins in 11Z cells. E GST-pull down assay analysis of CHIP and HMGB1 proteins interaction using purified GST-HMGB1 and His-CHIP. F Confocal immunofluorescence microscopy was performed to analyze localization of CHIP (red) and HMGB1 (green) in endometriotic cells