Extended Data Table 1 |.
Cryo-EM data collection, refinement and validation statistics
| SSTR2/Octreotide/Gi3/scFv16 | SSTR2/SST14/Gi3/scFv16 | |
|---|---|---|
| Data collection and processing | ||
| Magnification (x) | 57,050 | 57,050 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e−/Å2) | 67.00 | 52.00 |
| Defocus range (μm) | −0.8 to −1.8 | −0.8 to −1.8 |
| Pixel size (Å) | 0.8521 | 0.8521 |
| Symmetry imposed | C1 | C1 |
| Initial particle images (no.) | 6,860,866 | 4,464,832 |
| Final particle images (no.) | 281,479 | 442,863 |
| Map resolution (Å) | 2.9 | 2.5 |
| FSC threshold | 0.143 | 0.143 |
| Refinement | ||
| Model Resolution | 2.9 | 2.5 |
| FSC Threshold | 0.143 | 0.143 |
|
Model Composition Non-hydrogen Atoms |
8215 | 8428 |
| Protein Atoms | 8215 | 8423 |
| Waters & Ions B factor (Å2) |
0 | 5 |
| Protein Atoms | 46.84 | 52.86 |
| Waters & Ions | 49.20 | |
|
R.M.S Deviations Bonds (Å) |
0.005 | 0.005 |
| Angles (º) | 0.907 | 0.997 |
|
Validation MolProbity score |
1.58 | 1.33 |
| Clashscore | 5.26 | 3.49 |
| Poor rotamers (%) | 0.13 | 0.73 |
|
Ramachandran Plot Favored (%) |
95.69 | 96.89 |
| Allowed (%) | 4.31 | 3.02 |
| Outliers (%) | 0.00 | 0.09 |