Table 1. Yeast two-hybrid assays.
| DNA-binding domain | Activation domain plasmid | Growth |
|---|---|---|
| (pBTM116) | (pACT2) | (colony formation) |
| RPA32 | – | |
| RPA32 | – | |
| RPA32 | RPA14 | ++++ |
| RPA14 | RPA32 | ++++ |
| XPA | ++++ | |
| XPA | – | |
| XPA | RPA32 | ++++ |
| RPA32 | XPA | +++ |
| UDG | – | |
| UDG | – | |
| UDG | RPA32 | +++ |
| RPA32 | UDG | +++ |
| UDG | RBT1 | – |
| RBT1 | +++++ | |
| RBT1-ΔC16 | ++++ | |
| RBT1-ΔC36 | +++ | |
| RBT1-ΔC57 | ++ | |
| RBT1-ΔC79 | – | |
| RBT1 (pAS2-1; strain Y190) | +++++ | |
| RBT1 | – | |
| RBT1-ΔC16 | – | |
| RBT1-ΔC36 | – | |
| RBT1-ΔC57 | – | |
| RBT1-ΔC79 | – | |
| RBT1 | RPA32 | ++++ |
| RPA14 | RBT1 | – |
| RPA32 | RBT1 | +++ |
| RPA32 | RBT1-ΔC16 | +++ |
| RPA32 | RBT1-ΔC36 | +++ |
| RPA32 | RBT1-ΔC57 | +++ |
| RPA32 | RBT1-ΔC79 | +++ |
Growth on histidine deficient media containing 25 mM 3-amino-1,2,4-triazole determined qualitatively by vigour of colony formation. Where two proteins are assayed for interaction, the selection media is also deficient for tryptophan, leucine and histidine. Where a protein is assayed for activation of the HIS3 reporter gene by itself, the media is deficient for either tryptophan or leucine and histidine. Plus signs refer to how quickly and the vigour with which the resultant colonies grow.