Table 3.

Second experiment: Linear mixed model on embryo hatching time, hatchling length, larval length, and yolk sac volume at 130 dpf when predicted by treatment (exposure to Aeromonas salmonicida) and paternal skin coloration (yellowness) and/or parental kinship.

Effects	Hatching time				Hatchling length				Larval length 130dpf				Yolk sac volume 130dpf
Fixed effects	Coefficients	DF	t	p	Coefficients	DF	t	p	Coefficients	DF	t	p	Coefficients	DF	t	p
Intercept	93.74 ± 0.40	172.7	235.5	<0.001	7.15 ± 0.32	2614	22.6	<0.001	21.52 ± 0.42	2254	50.9	<0.001	−25.89 ± 4.34	2417	−5.97	<0.001
Treatment	−0.32 ± 0.07	161.8	−4.89	<0.001	−0.10 ± 0.008	165.6	−13.2	<0.001	−0.10 ± 0.01	168.6	−10.8	<0.001	0.02 ± 0.11	164.1	0.20	0.84
Yellowness	−0.01 ± 0.01	172.3	−1.16	0.25	−0.0003 ± 0.001	169.9	−0.17	0.86	0.002 ± 0.002	168.8	0.90	0.37	0.02 ± 0.02	173.2	1.03	0.31
Kinship	−7.30 ± 2.40	170	−3.04	0.003	−0.33 ± 0.33	167.2	−0.98	0.33	−0.70 ± 0.46	164.4	−1.51	0.13	−11.65 ± 4.00	170.5	−2.92	0.004
Yellowness × kinship	0.46 ± 0.28	173.3	1.67	0.10	0.01 ± 0.04	170.4	0.33	0.74	0.02 ± 0.05	168.6	0.30	0.76	−0.53 ± 0.46	173.4	−1.15	0.25
Treatment ×yellowness	−0.007 ± 0.005	160.7	−1.28	0.20	0.002 ± 0.001	160	2.81	0.006	0.0001 ± 0.0001	161.5	0.13	0.90	0.01 ± 0.01	156.5	1.27	0.21
Treatment × kinship	0.62 ± 1.14	152.8	0.54	0.59	−0.13 ± 0.13	145.2	−1.00	0.32	−0.04 ± 0.16	142.7	−0.25	0.80	1.31 ± 1.82	144.8	0.72	0.47
Hatching time					0.06 ± 0.003	2745	17.3	<0.001	−0.06 ± 0.004	2299	−12.6	<0.001	0.58 ± 0.05	2513	12.79	<0.001
Random effectsa	Variance components				Variance components				Variance components				Variance components
Family ID	1.62 ± 0.27			<0.001	0.03 ± 0.005			<0.001	0.07 ± 0.01			<0.001	4.39 ± 0.72			<0.001
Family × treatment	1.01 ± 0.16			<0.001	0.003 ± 0.002			0.14	0.003 ± 0.003			0.40	0.80 ± 0.43			0.06
Residual	4.13 ± 0.11				0.16 ± 0.004				0.20 ± 0.006				24.11 ± 0.69

Hatching time was included as a further covariate when testing for effects on hatchling length. The table gives the parameters as in Table 1. Family identity and the interaction between family ID and treatment were included as random factors. Significant p-values are highlighted in bold.

^aREML unbounded variance components ± standard error, Wald p-values.