Table 5. Ranking order of gene expression stability.
| Method | 1st |
2nd |
3rd |
4th |
5th |
6th |
7th |
|||||||
| Control | SIDR | Control | SIDR | Control | SIDR | Control | SIDR | Control | SIDR | Control | SIDR | Control | SIDR | |
| Delta-Ct | 18s | 18s | HPRT | HPRT | 36B4 | GAPDH | CycA | ActB | GAPDH | 36B4 | U6 | U6 | ActB | CycA |
| BestKeeper | 36B4 | 18s | 18s | ActB | HPRT | U6 | CycA | GAPDH | GAPDH | HPRT | U6 | 36B4 | ActB | CycA |
| NormFinder | HPRT | 18s | 18s | GAPDH | 36B4 | HPRT | CycA | ActB | GAPDH | 36B4 | U6 | U6 | ActB | CycA |
| geNorm | CycA/36B4 | 18s/HPRT | - | - | HPRT | GAPDH | 18s | U6 | GAPDH | ActB | U6 | 36B4 | ActB | CycA |
| Recommended comprehensive ranking | 36B4 | 18s | 18s | HPRT | HPRT | GAPDH | CycA | ActB | GAPDH | U6 | U6 | 36B4 | ActB | CycA |
Ranking order (better-good-average). SIDR: Streptozotocin-induced diabetic rats; 18s: 18s ribosomal unit 5; CycA: Cyclophilin A; 36B4: Ribosomal protein large P0; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; HPRT: Hypoxanthine-guanine phosphoribosyl transferase; ActB: Beta-actin; U6: Terminal uridylyl transferase 1.