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Fluorescence microscopy of MSC as monolayer or on collagen matrix. Cells were incubated with 10 ng/ml TGF-β3 and/or 10 μM Y-27632 and analyzed at 24 h and 72 h. Representative images show scleraxis immunostaining (24 h), phalloidin staining of the actin cytoskeleton and nuclear staining with DAPI (scalebar 50 µm).
