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. 2024 May 6;14:10393. doi: 10.1038/s41598-024-60717-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot results of Smad2/3 linker phosphorylation. Cells cultured as monolayer or on collagen matrix were incubated with 10 ng/ml TGF-β3 and/or 10 μM Y-27632. Representative Western blot result of linker phosphorylation at Ser^245/250/255 of Smad2 after 24 h (A) and 30 min (B) stimulation and at Ser²⁰⁴ of Smad3 after 30 min stimulation (C). Quantitative analysis of linker phosphorylation at Ser^245/250/255 (B: n = 5; D: n = 3) and Ser²⁰⁴ (C, n = 4). Box plots represent the ratio of P-Smad to Smad2/3 and dots the values of the different donors.