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. 2024 May 6;14:10393. doi: 10.1038/s41598-024-60717-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression of ITGB1 (A) and ROCK activity assay (B). (A) Cells cultured as monolayer or on collagen matrix were incubated with 10 ng/ml TGF-β3 and/ or 10 μM Y-27632. Box plots represent the relative gene expression of ITGB1. Asterisks mark differences between the stimulation groups with p < 0.05. Letters mark differences with p < 0.05 between collagen matrix and monolayer within same stimulation groups or in comparison to monolayer control (n = 4). (B) wild type (WT) and CD29^−/− cells were cultured as monolayer or on collagen matrix and analyzed after 24, 48 and 72 h. Box plots represents the ROCK activity. Asterisks mark differences between the WT and CD29^−/− with p < 0.05 and letters differences between the different timepoints sharing the same letter with p < 0.05 (n = 3).