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. 2024 Jan 20;39(2):335–337. doi: 10.1016/j.virs.2024.01.004

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© 2024 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 1 — Isolation and characterization the MPXV clinical isolate SZTH42. A MPXV proliferation induced cytopathic effects of in Vero E6 cells. White arrows indicate plaques. B Phylogenetic analysis of representative MPXV isolates from China in 2023 using Nextclade (v2.14.1) online software. C Immunofluorescence analysis of Vero E6 cell infected with MPXV SZTH42 isolate using rabbit derived VACV polyclonal antibody (pAb) or rabbit IgG (negative control). D Quantification of infectious MPXV from the third passage of SZTH42 isolation using focus forming assay in 96-well plate in duplicate. E Vero E6 cells in 24-well plates were infected with the SZTH42 isolate (MOI 0.01) in triplicate. The cell culture media and cell lysate were collected separately at indicated timepoints for titration of infectious virus particles using focus forming assay. Data was represented as mean ± SD. F Neutralizing activity of plasma of mpox patients against MPXV SZTH42 isolate was determined in Vero E6 cells using FRNT assay. The plasma from a healthy donor served as a negative control (HC). Day: time from admission to blood sampling; ID50: half maximal inhibitory dilution.