Table 1.
Summary of viruses identified from H. longicornis tick pools by RNA-seq and their TPM values and identities compared with reference viruses.
| Event | Reference virus | Family | Coverage (%) | Identity (%) | TPM |
|---|---|---|---|---|---|
| 1 | Bole tick virus 3 | Chuviridae | 24.39–98.63 | 41.7–80.6 | 10.98 |
| 2 | Changping tick virus 2 | 18.25–91.53 | 48.7–83.1 | 5.35 | |
| 3 | Chuviridae sp. | 12.46–17.41 | 81.1–82.8 | 8.16 | |
| 4 | Hebei mivirus 2 | 35.45–95.19 | 48.9–79.5 | 0.46 | |
| 5 | Karukera tick virus | 15.56–53.85 | 54.0–85.3 | 0.63 | |
| 6 | Wuhan tick virus 2 | 57.68–100.0 | 94.4–99.4 | 13.78 | |
| 7 | Xinjiang mivirus 1 | 40.33 | 80.0 | 0.63 | |
| 8 | Huangpi tick virus 1 | Nairoviridae | 5.4–93.14 | 95.5–99.8 | 28.68 |
| 9 | Taggert virus | 14.11–86.84 | 86.7–86.7 | 2.90 | |
| 10 | Granville quaranjavirus | Orthomyxoviridae | 60.98–96.22 | 61.9–83.6 | 64.07 |
| 11 | Ohshima virus | 32.77–94.8 | 27.4–48.4 | 1.90 | |
| 12 | Zambezi tick virus 1 | 30.21–92.82 | 44.9–76.4 | 36.89 | |
| 13 | Lone star tick densovirus 1 | Parvoviridae | 24.31–99.0 | 31.6–58.5 | 8.12 |
| 14 | Dabieshan tick virus | Phenuiviridae | 22.63–100.0 | 58–100 | 30658.97 |
| 15 | Kismayo virus | 31.15–99.44 | 43.8–47.9 | 1.80 | |
| 16 | Lihan tick virus | 99.66 | 99.4 | 1.75 | |
| 17 | Lone star virus | 32.45–71.53 | 50–52.2 | 1.13 | |
| 18 | Okutama tick virus | 44.8–99.27 | 99.3–99.7 | 839.31 | |
| 19 | Rhipicephalus associated phlebovirus 1 | 97.32–98.67 | 93.8–96.7 | 2.61 | |
| 20 | Huangpi tick virus 3 | Rhabdoviridae | 11.83–98.98 | 35.0–58.9 | 2.35 |
| 21 | IRE/CTVM19 associated rhabdovirus | 14.41–89.05 | 52.6–70.2 | 1.99 | |
| 22 | Tacheng tick virus 3 | 48.14–52.91 | 58.5–58.8 | 0.78 | |
| 23 | Wuhan tick virus 1 | 62.82 | 99.0 | 3.01 | |
| 24 | Lonestar tick totivirus | Totiviridae | 15.06–99.69 | 33.3–70.7 | 15.81 |
| 25 | Xinjiang tick totivirus 2 | 33.16–99.5 | 40.5–71.2 | 2.46 | |
| 26 | Alongshan virus | Unclassified viruses | 20.18–99.7 | 65.4–100 | 18.26 |
| 27 | Jingmen tick virus | 8.25–99.96 | 61.8–100 | 735.60 | |
| 28 | Bole tick virus 4 | 13.97–95.33 | 76.2–85.7 | 0.72 | |
| 29 | Henan tick virus | 29.39–91.23 | 97.1–99.4 | 83.81 | |
| 30 | Hepelivirales sp. | 24.11–99.81 | 67.6–100 | 396.84 | |
| 31 | Hubei sobemo like virus 15 | 8.45–99.85 | 57.0–100 | 7.60 | |
| 32 | Hubei toti like virus 24 | 34.95–99.75 | 31.5–71.3 | 4.95 | |
| 33 | Ixodes scapularis associated virus 2 | 13.65–87.34 | 45.9–76.7 | 3.94 | |
| 34 | Liman tick virus | 17.08–99.66 | 36.3–68.7 | 11.63 | |
| 35 | Lone star tick associated virus-1 | 8.45 | 52.7 | 27.62 | |
| 36 | Manly virus | 37.55–99.67 | 29.9–75.0 | 6.91 | |
| 37 | Norway mononegavirus 1 | 30.43–99.64 | 27.3–79.4 | 0.76 | |
| 38 | Shanxi tick virus 1 | 55.22 | 99.2 | 1.03 | |
| 39 | Tick borne tetravirus-like virus | 88.41–95.68 | 91.2–100 | 9.89 | |
| Total TPM | 33024.07 | ||||