Table 1.
Comparison of detection methods for fungus and bacteria.
| Microorganisms | Detection methods | Sample case (n) | Positive case (n) | Positive rate (%) |
|---|---|---|---|---|
| Fungi | SC | 143 | 99 | 69.2 |
| CM | 137 | 113 | 82.5 | |
| mNGS | 70 | 51 | 72.9 | |
| PS | 109 | 40 | 36.7 | |
| χ 2 | 60.436 | |||
| p | <0.001 | |||
|
| ||||
| Bacteria | SC | 67 | 46 | 68.7 |
| mNGS | 38 | 34 | 89.5 | |
| χ 2 | 5.792 | |||
| p | 0.016 | |||
SC, secretion culture; CM: corneal laser confocal microscopy; PS: pathological section; mNGS, metagenomic next-generation sequencing. For fungus: the positive rate of CM was higher than that of SC (χ2 = 6.681, p=0.010), but no difference was observed between CM and mNGS (χ2 = 2.608, p=0.106), the positive rate of SC was similar to mNGS (χ2 = 0.297, p=0.586), and the positive rate of PS was the lowest (all p < 0.05). For bacteria, 21 SC cases were negative but were detected using mNGS. Four cases of mNGS were negative but were detected using SC.