Skip to main content
. Author manuscript; available in PMC: 2024 May 7.
Published in final edited form as: Cell Rep. 2020 Aug 4;32(5):107981. doi: 10.1016/j.celrep.2020.107981

Figure 3. Dual Targeting of CD4bs and FP Yields Consistent CD4bs-Directed and Sporadic FP-Directed Neutralizing Responses.

Figure 3.

(A) Immunization schema. Key time points for analysis are indicated with colored arrows.

(B and D) ELISA IgG endpoint serum titers for binding FP (B) or BG505 trimer (D). Vertical dashed lines represent immunizations.

(C and E) Comparison of the three groups for serum endpoint titer to FP (C) or BG505 trimer (E) at three key time points.

(F) Neutralization ID50 titers of week 10 and 18 sera on CH505 CD4bs-degly4 virus. Horizontal dashed line indicates minimum detection level.

(G) Comparison of the three groups on serum neutralization against BG505 Δ611 over the course of the study.

(H) Neutralization ID50 titers on the 10-virus panel and BG505 D611 at the end of the study. This 10-strain panel was chosen as a sensitive means to detect neutralization by FP-targeting antibodies (Xu et al., 2018). Geometric mean ± 95% CI are shown. p values were calculated with Mann-Whitney two-tailed t test: *p < 0.05. ND, virus tier status has not been determined. See also Figures S2–S6 and Tables S1 and S3.