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. 2024 Mar 15;27(4):109512. doi: 10.1016/j.isci.2024.109512

Figure 5.

Figure 5

Inhibition of LMTK3 enhances KCC2 activity and S940 phosphorylation

(A) Representative traces are shown for the polarity of currents induced by rapid application of muscimol in DGGCs in slices from WT mice loaded with 32-mM Cl− at differing voltages, which had been pretreated with V or 10-μM C28 for 1 h prior to recording. The recordings were used to determine EGABA and [Cl−], which were then compared between treatments. ∗p < 0.01; t test; n = 5–6 mice.

(B) Representative traces are shown for the polarity of currents induced by rapid application of muscimol in DGGCs in slices from LMTK3-KO mice loaded with 32-mM Cl− at differing voltages, which had been pretreated with V or 10-μM C28 for 1 h prior to recording. The recordings were used to determine EGABA and [Cl−], which were then compared between treatments. n = 5–6 mice.

(C) Acute hippocampal slices from WT mice were exposed to V or C28. SDS-soluble lysates were then immunoblotted with KCC2, pS940, pT1007, or actin antibodies. The ratio of pS940/KCC2 and pT1007/KCC2 immunoreactivity were then determined and normalized to values seen in V. ∗p < 0.05; t test; n = 4 mice. In all panels data represent mean ± SEM.