Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 21;15(5):364–384. doi: 10.1093/procel/pwad063

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023. Published by Oxford University Press on behalf of Higher Education Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Characterization of the senescence hotspot and its microenvironment in aged ovaries. (A) Pseudotime analysis of GC, TC, and MT2^high spot in NHP ovary. Upper left, pseudotime scores of the three spot types in monkey ovary. Upper right, the distribution of young and old groups along the pseudotime trajectory. Middle left, the distribution of these three spot types along the pseudotime trajectory. Middle right, the distribution of different states of MT^high spot along the pseudotime trajectory. Lower left, bar plot showing the proportion distribution of three states of MT2^high spots between young and old groups. Lower right, the spatial distribution of three states of MT2^high spots. (B) Heatmap showing four different expression patterns along the pseudotime trajectory based on branched expression analysis modeling (BEAM) analysis and bar plot showing the enriched GO terms for cluster 1 genes. (C) Enriched GO terms and pathways of aging DEGs in MT2^high spot. (D) Bar plot showing pathway enrichment analysis of overlapped genes between upregulated aging DEGs in MT2^high spot and Cluster1 genes. (E) Heatmap showing the expression levels of the top 10 (LogFC) overlapping genes between upregulated aging DEGs in MT2^high spot and Cluster1 genes. (F) MT2 immunostaining in young and old monkey ovaries. The relative intensity was quantified as fold changes (old vs. young), and presented as mean ± SEMs on the right. Scale bars, 20 μm and 10 μm (zoomed-in images). n = 4 monkeys for each group. (G) Dot plot showing the interaction pairs between MT2^high and other spot types unique in the aged group.