KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-BDBV GP antiserum (FS0510) | This paper | N/A |
| Mouse anti-MARV GP monoclonal antibody (AGP127-8) | Kajihara et al., 2012 | N/A |
| Mouse anti-VSV G monoclonal antibody (VSV-G[N] 1-9) | Nakayama et al., 2011 | N/A |
| Mouse anti-EBOV GP monoclonal antibody (ZGP42/3.7) | Furuyama et al., 2016 | N/A |
| Mouse anti-EBOV NP monoclonal antibody (ZNP74-7) | Changula et al., 2013 | N/A |
| Rabbit anti-MARV GP antisera (FS0505) | Furuyama et al., 2016 | N/A |
| Rabbit anti-MARV NP antisera (FS0608) | Furuyama et al., 2016 | N/A |
| Goat anti-mouse IgG polyclonal antibody conjugated with FITC | Jackson ImmunoResearch | Cat#115-095-003; RRID:AB_2338589 |
| Donkey anti-rabbit IgG polyclonal antibody conjugated with FITC | Jackson ImmunoResearch | Cat#711-096-152; RRID:AB_2340597 |
| Rabbit anti-MARV NP antisera (FS0609) | Furuyama et al., 2016 | N/A |
| Goat anti-rabbit IgG polyclonal antibody conjugated with Alexa Fluor 488 | Invitrogen | Cat#A11034; RRID:AB_2576217 |
| Rat anti-HA monoclonal antibody | Sigma | Cat#11867423001; RRID:AB_390918 |
| Goat anti-rat IgG (H+L) polyclonal antibody conjugated with horseradish peroxidase | Jackson ImmunoResearch | Cat#112-035-003; RRID:AB_2338128 |
| Rabbit anti-NPC1 monoclonal antibody | Abcam | Cat#ab134113; RRID:AB_2734695 |
| Mouse anti-β actin monoclonal antibody | Abcam | Cat#ab6276; RRID:AB_2223210 |
| Goat anti-rabbit IgG polyclonal antibody conjugated with horseradish peroxidase | KPL | Cat#074-1506; RRID:AB_2721169 |
| Goat anti-mouse IgG polyclonal antibody conjugated with horseradish peroxidase | Jackson ImmunoResearch | Cat#115-035-062; RRID:AB_2338504 |
| Donkey anti-rabbit IgG (H+L) polyclonal antibody conjugated with Alexa Fluor 488 | Invitrogen | Cat#A21206; RRID:AB_141708 |
| Rabbit anti-LAMP1 polyclonal antibody | Sigma-Aldrich | Cat#SAB3500285; RRID:AB_10646194 |
| Bacterial and Virus Strains | ||
| VSV, whose genome code the GFP gene instead of the G protein gene | Takada et al., 1997 | N/A |
| EBOV (Mayinga) | The University of Texas Medical Branch, TX, USA | N/A |
| MARV (Musoke) | The University of Texas Medical Branch, TX, USA | N/A |
| EBOV-GFP (Mayinga) | Ebihara et al., 2007 | N/A |
| MARV (Angola) | NIAID/NIH, MT, USA | N/A |
| Deposited Data | ||
| NPC1 sequence of FBKT1 | This paper | GenBank: LC462999 |
| NPC1 sequence of ZFBK13-76E | This paper | GenBank: LC462999w |
| NPC1 sequence of ZFBK11-97 | This paper | GenBank: LC462994 |
| NPC1 sequence of ZFBK15-137RA | This paper | GenBank: LC462995 |
| NPC1 sequence of DemKT1 | This paper | GenBank: LC462996 |
| NPC1 sequence of SuBK12-08 | This paper | GenBank: LC462997 |
| NPC1 sequence of YubFKT1 | This paper | GenBank: LC462271 |
| NPC1 sequence of BKT1 | This paper | GenBank: LC462998 |
| Experimental Models: Cell Lines | ||
| Vero E6 | ATCC | CRL-1586 |
| HEK293T | ATCC | CRL-1573 |
| FBKT1 | Maeda et al., 2008 | N/A |
| ZFBK13-76E | Ogawa et al., 2017 | N/A |
| ZFBK11-97 | Maruyama et al., 2014 | N/A |
| ZFBK15-137RA | Sato et al., 2019 | N/A |
| DemKT1 | Maeda et al., 2008 | N/A |
| SuBK12-08 | Maruyama et al., 2014 | N/A |
| YubFKT1 | Maeda et al., 2008 | N/A |
| BKT1 | Maeda et al., 2008 | N/A |
| FBKT1-HEK293T NPC1 | This paper | N/A |
| FBKT1-empty vector | This paper | N/A |
| ZFBK13-76E-HEK293T NPC1 | This paper | N/A |
| ZFBK13-76E-empty vector | This paper | N/A |
| Vero E6/NPC1-KO cl.19 HEK293T NPC1 | Kondoh et al., 2018 | N/A |
| Vero E6/NPC1-KO cl.19 | Kondoh et al., 2018 | N/A |
| Vero E6/NPC1-KO cl.19 empty vector | Kondoh et al., 2018 | N/A |
| Vero E6/NPC1-KO cl.19 HEK293T NPC1/TET | This paper | N/A |
| Vero E6/NPC1-KO cl.19 FBKT1 NPC1 | This paper | N/A |
| Vero E6/NPC1-KO cl.19 FBKT1 NPC1/SGA | This paper | N/A |
| Vero E6/NPC1-KO cl.19 HEK293T NPC1/FT | This paper | N/A |
| Vero E6/NPC1-KO cl.19 ZFBK13-76E | This paper | N/A |
| Vero E6/NPC1-KO cl.19 ZFBK13-76E/DV | This paper | N/A |
| Vero E6/NPC1-KO cl.19 HEK293T NPC1/G426E | This paper | N/A |
| Vero E6/NPC1-KO cl.19 FBKT1 NPC1/E426G | This paper | N/A |
| Vero E6/NPC1-KO cl.19 HEK293T NPC1/D502F | This paper | N/A |
| Vero E6/NPC1-KO cl.19 ZFBK13-76E NPC1/F502D | This paper | N/A |
| Platinum-GP cells | Cell Biolabs | RV-103 |
| Recombinant DNA | ||
| pCAGGS-EBOV GP | Changula et al., 2013 | N/A |
| pCAGGS-SUDV GP | Changula et al., 2013 | N/A |
| pCAGGS-TAFV GP | Changula et al., 2013 | N/A |
| pCAGGS-BDBV GP | Changula et al., 2013 | N/A |
| pCAGGS-RESTV GP | Changula et al., 2013 | N/A |
| pCAGGS-MARV GP | Changula et al., 2013 | N/A |
| pCAGGS-EBOV GP S142Q | This paper | N/A |
| pCAGGS-SUDV GP Q142S | This paper | N/A |
| pCAGGS-TAFV GP S142Q | This paper | N/A |
| pCAGGS-BDBV GP S142Q | This paper | N/A |
| pCAGGS-RESTV GP Q143S | This paper | N/A |
| pCAGGS-MARV GP Q126S | This paper | N/A |
| pCAGGS-EBOV GP A148P | This paper | N/A |
| pCAGGS-SUDV GP P148A | This paper | N/A |
| pCAGGS-TAFV GP P148A | This paper | N/A |
| pCAGGS-BDBV GP P148A | This paper | N/A |
| pCAGGS-RESTV GP P149A | This paper | N/A |
| pCAGGS-MARV GP A132P | This paper | N/A |
| pCAGGS-EBOV GP V79P | This paper | N/A |
| pCAGGS-EBOV GP P80L | This paper | N/A |
| pCAGGS-EBOV GP T83S | This paper | N/A |
| pCAGGS-EBOV GP G87A | This paper | N/A |
| pCAGGS-EBOV GP L111I | This paper | N/A |
| pCAGGS-EBOV GP E112S | This paper | N/A |
| pCAGGS-EBOV GP I113V | This paper | N/A |
| pCAGGS-EBOV GP K114T | This paper | N/A |
| pCAGGS-EBOV GP V141I | This paper | N/A |
| pCAGGS-EBOV GP T144Q | This paper | N/A |
| pCAGGS-EBOV GP G145N | This paper | N/A |
| pCAGGS-EBOV GP C147H | This paper | N/A |
| pCAGGS-EBOV GP I170M | This paper | N/A |
| pCAGGS-MARV GP P63V | This paper | N/A |
| pCAGGS-MARV GP L64P | This paper | N/A |
| pCAGGS-MARV GP S67T | This paper | N/A |
| pCAGGS-MARV GP A71G | This paper | N/A |
| pCAGGS-MARV GP I95L | This paper | N/A |
| pCAGGS-MARV GP S96E | This paper | N/A |
| pCAGGS-MARV GP V97I | This paper | N/A |
| pCAGGS-MARV GP T98K | This paper | N/A |
| pCAGGS-MARV GP I125V | This paper | N/A |
| pCAGGS-MARV GP Q128T | This paper | N/A |
| pCAGGS-MARV GP N129G | This paper | N/A |
| pCAGGS-MARV GP H131C | This paper | N/A |
| pCAGGS-MARV GP M154I | This paper | N/A |
| pCAGGS-EBOV VP40 | Changula et al., 2013 | N/A |
| pCAGGS-EBOV NP | Changula et al., 2013 | N/A |
| pCAGGS-MARV VP40 | Changula et al., 2013 | N/A |
| pCAGGS-MARV NP | Changula et al., 2013 | N/A |
| pMXs-puro-HEK293T NPC1 | Kondoh et al., 2018 | N/A |
| pMXs-puro-HEK293T NPC1/TET | This paper | N/A |
| pMXs-puro-FBKT1 NPC1 | This paper | N/A |
| pMXs-puro-FBKT1 NPC1/SGA | This paper | N/A |
| pMXs-puro-ZFBK13-76E NPC1 | This paper | N/A |
| pMXs-puro-ZFBK13-76E NPC1/DV | This paper | N/A |
| pMXs-puro-HEK293T NPC1/G426E | This paper | N/A |
| pMXs-puro-FBKT1 NPC1/E426G | This paper | N/A |
| pMXs-puro-HEK293T NPC1/D502F | This paper | N/A |
| pMXs-puro-ZFBK13-76E NPC1/F502D | This paper | N/A |
| Software and Algorithms | ||
| PyMOL | Schrödinger LLC | https://pymol.org/2/ |
| R software (version 3.5.2) | R Core Team, 2018 | https://cran.r-project.org/ |
| ZEN 2.3 Lite | Carl Zeiss | https://www.zeiss.co.jp/microscopy/products/microscope-software/zen-lite.htm |