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. 2023 Dec 14;20(6):229–235. doi: 10.1089/zeb.2023.0054

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© Kazumasa Takemoto et al. 2023; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 1. — Effect of FBS, BSA, and Glu on the fertility of stored sperm. Pooled sperm from several males were stored in L-15 containing PS with/without FBS, BSA, and Glu at 4°C for 4 days, and used to fertilize 100–200 oocytes. (A) Effect of FBS (n = 8). (B) Effect of BSA (n = 6–7). (C) Effect of Glu (n = 5). The shape of the marks indicates the same sampling batch of pooled sperm. The mean was calculated by combining all results. Error bars indicate the standard deviation. *P ≤ 0.05, ****P ≤ 0.0001. BSA, bovine serum albumin; FBS, fetal bovine serum; Glu, glucose; PS, penicillin/streptomycin.