Figure 2. Vitamin C is imperative to activate recombinant TET2 in our system. (.

A) Similar to Fig. 1b, bar graphs indicating the oxidation state of methylated DNA over a time course, revealing that vitamin C is crucial to TET2 function. Each reaction was supplemented with Fe²⁺ and/or VC as indicated. (B) Similar to Fig. 1e, line graphs of αKG and succinate levels over time during reactions indicated in (A). (C-D) DNA increases the rate of conversion of αKG to succinate, compared to the uncoupled reactions without DNA, monitored by NMR. Reactions were monitored without DNA (left), with unmethylated DNA (center), or with methylated DNA (right) in the absence (C) or presence (D) of VC. Experiments were performed in at least duplicate. Errors bars: SD.