Fig 1.

E. faecalis disseminate from the colon to the cMLN. (A) Experimental timeline for ceftriaxone-induced E. faecalis dissemination model. (B) The cMLN and siMLNs were collected separately, homogenized, and plated on selective agar to enumerate E. faecalis dissemination. (C) Colon contents were collected and plated to enumerate colonic E. faecalis abundance (x-axis) and plotted against cMLN dissemination (y-axis). (D) Experimental timeline for germ-free E. faecalis dissemination model. (E) E. faecalis dissemination to the cMLN and siMLN of germ-free mice was quantified as in A. (B and E) Median and interquartile range are reported, and P values were calculated using a Mann-Whitney test. Data are pooled from three independent experiments, using three to five mice each. (C) Data are pooled from four independent experiments using five mice per group. R² and P values were calculated using a Pearson correlation analysis. LOD, limit of detection (10 CFU/organ); P > 0.05 not reported.