Fig 2.

Clodronate-mediated depletion of colonic phagocytes reduces E. faecalis dissemination. (A) Experimental timeline for intracolonic liposome administration within our standard E. faecalis dissemination model. (B) Flow cytometric analysis of colonic non-B cell APCs (CD45+ CD3− CD19− MHCII+) or relevant APC subsets (C) as a percentage of CD45+ cells obtained from control (Ctrl) and clodronate-treated mice. (D) Colon contents from control or clodronate-treated mice were plated on selective agar to enumerate E. faecalis abundance. (E) MLNs from control or clodronate-treated mice were homogenized and plated on selective agar to enumerate E. faecalis dissemination. (B–D) Mean and standard error of the mean are reported, and statistical significance was determined using an unpaired t-test. Data are representative of three independent experiments using 5 mice (B and C) or 10 mice (D) per group. (E) Median and interquartile range are reported, and P values were calculated using a Mann-Whitney test. Data are pooled from three independent experiments, using four to five mice per group. LOD, limit of detection (10 CFU/organ); P > 0.05 not reported.