Fig 3.

Inhibiting DC recruitment to the MLNs does not have impact on E. faecalis dissemination. (A) Experimental timeline for anti-CCR7 (αCCR7) administration within our standard E. faecalis dissemination model. (B) Representative gating strategy for identifying non-B cell APCs in the MLN by flow cytometry. (C and D) Flow cytometric analysis of CCR7-expressing APCs (CD45+ CD3− CD19− MHCII+ CCR7+) within the MLNs of control (isotype) or αCCR7-treated mice. Y-axis represents the total cell number (C) or the percentage of CD45+ cells (D). (E) Effects of αCCR7 treatment on E. faecalis dissemination in ceftriaxone-treated mice. (C and D) Data are representative of two independent experiments, using five mice per group. Mean and standard error of the mean are reported. Statistical significance was determined using an unpaired t-test. (E) Data are pooled from three independent experiments, using five mice per group. Median and interquartile range are reported. Statistical significance was determined using a Mann-Whitney test. LOD, limit of detection (10 CFU/organ); P > 0.05 not reported.