Fig 4.

CCR2 antagonism reduces E. faecalis dissemination. (A) Experimental timeline for CCR2 antagonism (CCR2-a) within our standard E. faecalis dissemination model. (B and C) Flow cytometric analysis of colonic APCs (CD45+ CD3− CD19− MHCII+) and relevant APC subsets (D and E) obtained from Ctrl or CCR2-a-treated mice. Y-axis represents the total cell number (B and D) or the percentage of CD45+ cells (C and E). (F) Effects of CCR2-a on E. faecalis dissemination in ceftriaxone-treated mice. (B–E) Data are representative of three independent experiments, using five mice per group. (D) Data are pooled from two experiments to account for variability in cell yields. Mean and standard error of the mean are reported. Statistical significance was determined using an unpaired t-test. (F) Data are pooled from three independent experiments, using five mice per group. Median and interquartile range are reported. Statistical significance was determined using a Mann-Whitney test. LOD, limit of detection (10 CFU/organ); P > 0.05 not reported.