Fig 2.

Host cell invasion by UPEC does not require active host transcription or protein synthesis. BECs were treated with ActD (5 µg/mL), CHX (26 µM), or carrier (ethanol) alone for 30 min and then infected in the continued presence of the inhibitors with UTI89 for 2 h followed by a 2-h incubation in medium containing gentamicin. Graphs show levels of (A) host cell-associated bacteria and (B) intracellular, gentamicin-protected bacteria, with bars indicating mean values. Data from three independent experiments performed in triplicate are expressed relative to controls that were treated with carrier (EtOH) alone. P values, as calculated by Student’s t tests, were all ≥0.28.