Fig 5.

FAK inhibition and deletion mirror the effects of CAPE and resveratrol on host cell invasion by UPEC. (A and B) BECs or (C–H) FAK+/+ and FAK−/− MEFs were treated with FAK14 (10 µg/mL), CAPE (25 µg/mL), resveratrol (22.9 µg/mL), carrier (DMSO) alone, or left untreated, as indicated, for 1 h prior to and during a 2-h infection with UTI89. Monolayers were then washed and processed to determine total numbers of host cell-associated bacteria or incubated for an additional 2-h period with gentamicin to eradicate extracellular bacteria. Graphs show relative levels of (A, C, E, and G) host cell-associated bacteria and (B, D, F, and H) intracellular, gentamicin-protected bacteria. Data were normalized to DMSO-treated controls or to wild-type (FAK+/+) MEFs, as applicable, with bars representing mean values from three independent experiments carried out in triplicate. P values were determined by Student’s t tests.