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Schematic outline of mRNA–peptide fusion synthesis. The mRNA was hybridized to a DNA linker carrying psoralen (gray circle) at the 5′-terminus and puromycin (Pu) at its 3′-end. Irradiation with UV light led to covalent crosslink formation between the linker and mRNA 3′-end. In vitro translation of the photo-crosslinked mRNA–puromycin template produced the mRNA–peptide fusion product.
