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In vitro translation and fusion formation. Protein synthesis from mRNA templates 2 and 3 is shown in lanes 1 and 3, respectively. Translation of mRNA templates photo-crosslinked to linker B produced mRNA–protein fusions (lanes 2 and 4). Template 3 carries a stop codon following the linker crosslink site. Lane 5 shows the fusion product after purification on oligo(dT)–cellulose. The products were analyzed by electrophoresis on a 4–12% NuPage gel using MES running buffer (Novex).
