Fig. 3.

FBF-2 interacts with CSN-5 in vivo. a–c) Confocal maximal projection images of the entire adult distal germline SPC zones with PLA foci (yellow) and DAPI (blue). Individual DAPI channels (aii, bii, and cii) are shown in grayscale for better contrast as maximal projections spanning half the depth of the germline. Germlines are outlined with dashed lines and vertical dotted lines indicate the beginning of the transition zone as indicated by DNA morphology. Genotypes are indicated with their respective images, where 3xflag::csn-5 transgenes were driven by the csn-5 promoter and gfp and gfp::fbf transgenes were driven by the gld-1 promoter. Scale bars: 10 µm. b) The PLA density (number of PLA foci per µm²) within the SPC zone was measured for germlines of each genetic background; each dot represents a single germline. Differences in PLA density for each protein pair were evaluated by 1-way ANOVA with Tukey's posttest. Asterisks denote statistical significance (****P < 0.0001) where FBF-2 had significantly greater PLA foci density than both FBF-1 and the control. Number of germlines scored (N) are indicated below the graph. Data are representative of 3 biological replicates. Mean group values are shown as lines and error bars denote SEM. All experiments were performed at 24°C.