Skip to main content
. 2024 Mar 1;227(1):iyae033. doi: 10.1093/genetics/iyae033

Fig. 8.

Fig. 8.

ned-8(RNAi) rescues FBF-2 levels along with SPC numbers and oogenesis in csn-5(lf). a, b) Peak levels of FBF-1 a) and FBF-2 b) in distal germlines determined by immunostaining of 3xv5::fbf-2(q932), referred to here as wild type, and 3xv5::fbf-2; csn-5(lf), referred to here as csn-5(lf) treated with control or ned-8(RNAi). Endogenous FBF-1 and epitope-tagged endogenous 3xV5::FBF-2 detected by anti-FBF-1 and anti-V5. Peak FBF protein intensity in each germline was normalized to the average of wild-type control RNAi after background subtraction. Differences in protein level between control and ned-8(RNAi) were evaluated by 1-way ANOVA with Sidak's posttest, ns denotes not significant, and red asterisks denote statistical significance (****P < 0.0001). Number of germlines scored (N) is indicated at the bottom of the graphs. Mean group values are shown as lines, and error bars denote standard deviation. The data reflect 3 biological replicates. c–f) Representative images of wild-type and csn-5(lf) mutant germlines dissected and stained with anti-FBF-1 and anti-V5 to visualize protein levels of FBF-1/-2. Germlines are outlined with dashed lines. Scale bars: 10 μm. g) Quantification of SPCs in wild-type and csn(lf) mutants with ned-8(RNAi); each data point represents a single germline. Differences in number of SPCs between control and ned-8(RNAi) were evaluated by 1-way ANOVA with Sidak's posttest. ns denotes not significant, and asterisks denote statistical significance (****P < 0.0001). Number of germlines scored (N) are indicated at the bottom of the graph. SPC quantification reflects 4 biological replicates of csn-5(lf) control RNAi and 3 biological replicates of wild-type control RNAi, wild-type ned-8(RNAi), and csn-5(lf) ned-8(RNAi). Mean group values are shown as lines, and error bars denote standard deviation. h) Gametogenesis in ned-8(RNAi)-treated wild-type and csn-5(lf) mutant. Number of germlines scored (N) are indicated at the top of each column. Data are plotted in aggregate and reflect 3 biological replicates.