Figure 2.
PDE8A (phosphodiestrase 8A) is in the vicinity of the AKAP12 (A kinase anchoring protein 12) signalosome in adult primary cardiomyocytes. A, Representative Western blot of left ventricular (LV) extracts from mice overexpressing AKAP12 (AKAP12OX) compared with wild-type (WT) littermates. B, Quantification of AKAP12 protein levels, N=4 in each group. C and D, ELISA assay comparing baseline cAMP (cyclic adenosine 3′,5′-monophosphate) levels in primary cardiomyocytes extracted from LVs of AKAP12OX males and female mice, respectively compared with WTs, N=3 in each group. E, Representative immunocytochemistry images of primary adult cardiomyocytes and their scatter plot for colocalization using Pearson’s correlation test. F, Quantification of PDE8A colocalization with AKAP12 in the absence of ISO; n=24 WT, n=7 AKAP12OX and in the presence of ISO; n=16 WT and n=8 AKAP12,OX pooled data. All data represented as average mean±SEM. Data were determined to have a parametric distribution by the Shapiro-Wilk test; α=0.05 and were analyzed using unpaired 2-tailed Student t test for all panels except for the AKAP12OX group in F which had nonparametric distribution, and data were compared within AKAP12OX group using Mann-Whitney U test. NC indicates negative controls.