Figure 3.

Overexpressing AKAP12 (A kinase anchoring protein 12) in adult primary cardiomyocytes reduces their contractility post acute isoproterenol (ISO) treatment. A and I, Average tracings of sarcomere length from all primary adult cardiomyocytes isolated from male and female mice and treated acutely with ISO. B through H, Quantification of contractility parameters among the AKAP12^OX and wild-type (WT) males, n=49 in WT and n=14-15 in AKAP12^OX. J through P, Quantification of contractility parameters among the AKAP12^OX and WT females, n=40-41 in WT and n=26 in AKAP12^OX. Comparison of paced cells parameters measured includes (B and J) diastolic sarcomere length (μm), (C and K) systolic sarcomere length (μm), (D and L) sarcomere shortening (%), (E and M) dl/dt- contraction (μm/s), (F and N) time to 90% peak (seconds), (G and O) dl/dt- relaxation (μm/s), and (H and P) time to 90% baseline (second). All data represented as average mean±SEM of median values for each animal except A and I, which represents the mean±SEM of all cells. N=4 in each group. Data were determined to have a parametric distribution by the Shapiro-Wilk test; α=0.005 and were analyzed using an unpaired 2-tailed Student t test. ‡‡ indicates that the isolation buffer contained Blebbistatin instead of BDM.