Figure 5.

PDE8 (phosphodiesterase 8) inhibitor (PF-04957325) reverses AKAP12^OX effect on [Ca²⁺]i and contractility in primary adult mice cardiomyocytes. A through C, Quantification of PDE8 inhibition effects on cardiomyocytes calcium and contractility and their scatter plot among the AKAP12^OX and wild-type (WT) males; n=35 in WT and n=12-13 in AKAP12^OX. D through F, Quantification of PDE8 inhibition effects on cardiomyocytes calcium and contractility and their scatter plot among the AKAP12^OX and WT females; n=27-29 in WT and n=20-21 in AKAP12^OX. N=3 to 4 in each group. When data were determined to have a parametric distribution by the Shapiro-Wilk test; α=0.05, AKAP12^OX groups in D and B, 1-way ANOVA was used to assess data within each group. Other data with nonparametric distribution were compared within each group using the Friedman test. Mann-Whitney U test was used to compare PF-04957325+ISO effects between groups in all except A; unpaired 2-tailed Student t test. ISO indicates isoproterenol; and PF; PF-04957325.