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. 2024 Apr 25;20(4):e1011237. doi: 10.1371/journal.pgen.1011237

Fig 6. Apical epidermal intercalation contributes to mechanical hypersensitivity.

Fig 6

(A-E) Neuronal integrin overexpression suppresses miR-14 mutant junctional dendrite alignment defect. Representative composite images show C4da dendrite arbors (white) and epidermal cell-cell junctions (magenta) for (A) wild-type control, (B) miR-14 mutant, (C) control larvae overexpressing UAS-mew and UAS-mys (UAS-Integrins) selectively in C4da neurons (ppk-GAL4), and (D) miR-14 mutant overexpressing UAS-Integrins selectively in C4da neurons. Hatched boxes indicate region of interest shown at high magnification to the right of each image. (E) Plot depicts the proportion of dendrite arbors aligned along epidermal junctions in larvae of the indicated genotypes. *P<0.05, ANOVA with post-hoc Tukey’s test. (F) Neuronal integrin overexpression has no effect on C4da neuron dendrite branch length or number. Plots depict the total dendrite length (left) and terminal dendrite number (right) normalized to segment size in wild-type control and miR-14 mutant larvae. Statistical tests (dendrite length, Fisher’s exact test; dendrite number, unpaired t-test with Welch’s correction) revealed no significant difference in either paramter (n = 7 neurons each). (G) Neuronal integrin overexpression suppresses miR-14 mutant mechanical hypersensitivity. Plot depicts nociceptive rolling responses of larvae of the indicated genotypes to different forces of von Frey stimulation. *P<0.05, Fisher’s exact test with a post-hoc BH correction. The number of larvae tested is shown for each condition.