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. 2024 May 7;19(5):e0301624. doi: 10.1371/journal.pone.0301624

Table 1. Primer and probe sequences for qPCR assays and sequencing.

Primer/Probe Sequence (5’ → 3’) Target Ref.
leadpGRG36 GGGGTGGAAATGGAGTTTTT pGRG36 McKenzie and Craig (18)
Tn7-F GATGCTGGTGGCGAAGCTGT tn7
Tn7-R GATGACGGTTTGTCACATGGA tn7
staG-Frt CGCGAAGTCAGAGTCGACATAG S. Typhi Nga, Karkey (17)
staG-Rrt AAGACCTCAACGCCGATCAC S. Typhi
staG-Probe a FAM-CATTTGTTCTGGAGCAGGCTGACGG-TAMRA S. Typhi
STmod-Probe HEX-GCACGAGATGTCTCAGTCCCGCATT-BHQ PCO This manuscript
ttr-F CTCACCAGGAGATTACAACATGG Pan Salmonella Nair, Patel (22)
ttr-R AGCTCAGACCAAAAGTGACCATC Pan Salmonella
ttr-Probe FAM-CACCGACGGCGAGACCGACTTT-BHQ1 Pan Salmonella
tviB-F TGTGGTAAAGGAACTCGGTAAA S. Typhi
tviB-R GACTTCCGATACCGGGATAATG S. Typhi
tviB-Probe TET-TGGATGCCGAAGAGGTAAGACGAGA-BHQ1 S. Typhi

a The LSTM staG probe contained Cy5 on the 5’ end rather than FAM.