Table 1. Primer and probe sequences for qPCR assays and sequencing.
Primer/Probe | Sequence (5’ → 3’) | Target | Ref. |
---|---|---|---|
leadpGRG36 | GGGGTGGAAATGGAGTTTTT | pGRG36 | McKenzie and Craig (18) |
Tn7-F | GATGCTGGTGGCGAAGCTGT | tn7 | |
Tn7-R | GATGACGGTTTGTCACATGGA | tn7 | |
staG-Frt | CGCGAAGTCAGAGTCGACATAG | S. Typhi | Nga, Karkey (17) |
staG-Rrt | AAGACCTCAACGCCGATCAC | S. Typhi | |
staG-Probe a | FAM-CATTTGTTCTGGAGCAGGCTGACGG-TAMRA | S. Typhi | |
STmod-Probe | HEX-GCACGAGATGTCTCAGTCCCGCATT-BHQ | PCO | This manuscript |
ttr-F | CTCACCAGGAGATTACAACATGG | Pan Salmonella | Nair, Patel (22) |
ttr-R | AGCTCAGACCAAAAGTGACCATC | Pan Salmonella | |
ttr-Probe | FAM-CACCGACGGCGAGACCGACTTT-BHQ1 | Pan Salmonella | |
tviB-F | TGTGGTAAAGGAACTCGGTAAA | S. Typhi | |
tviB-R | GACTTCCGATACCGGGATAATG | S. Typhi | |
tviB-Probe | TET-TGGATGCCGAAGAGGTAAGACGAGA-BHQ1 | S. Typhi |
a The LSTM staG probe contained Cy5 on the 5’ end rather than FAM.