Table 4. Average Cq and percent detection of the PCO in seeded lab studies at different concentrations.

The PCO was detected in all experiments when it was seeded at 10 CFU/mL or higher and was detected in all experiments when it was seeded at 1 CFU/mL for filter cartridge and differential centrifugation methods.

	1 CFU PCO/mL		10 CFU PCO/mL		100 CFU PCO/mL
	Positive Cq<40	Cq average	Positive Cq<40	Cq average	Positive Cq<40	Cq average
Membrane filtration	4/6 (66.7%)	37.9	16/16 (100%)	33.1	2/2 (100%)	30.3
Filter cartridge	6/6 (100%)	35.5	16/16 (100%)	31.5	2/2 (100%)	28.7
Recirculating Moore swab	4/6 (66.7%)	37.7	16/16 (100%)	32.7	2/2 (100%)	29.8
Differential centrifugation	3/3 (100%)	36.2	3/3 (100%)	32.6	-	-