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. 2024 Apr 22;18(4):e0012120. doi: 10.1371/journal.pntd.0012120

Fig 1. Genetic maps depicting the pMG5040 plasmid encoding the full-length rearranged RNA.

Fig 1

(a) Indicated are approximate locations of cytomegalovirus (CMV) promoter (solid arrow), 26S promoter (open arrows) and attenuating mutations (asterisks). The wild type CHIKV genome is shown in red. CHIKV pMG5040 plasmid encoded rearranged full-length infectious CHIKV RNA under transcriptional control of the CMV promoter. The plasmid pMG5040 contained both attenuating mutations (asterisks) from the IND vaccine 181/25. (b) 1% Agarose/TAE gel showing pMG5040 plasmid as compared to control VEEV vaccine. M, 1kB Plus DNA ladder (Thermo); 1, VEE control pMG4020: 2, CHIKV pMG5040.