Table 2. Rates at which selectable phenotypes are generated in ERCC1+ and ERCC1– cells.
Selection | Rates (×10–7) | |||
---|---|---|---|---|
ERCC1+ | ERCC1– | |||
GS22-15a | AK784b | GS22-13a | GSAK1b | |
TK–APRT– | 1.4 ± 0.5 | 1.1 | 6 ± 3 | 1.8 |
TK– | 15 ± 3 | 17 | 48 ± 23 | 62 |
APRT– | 14 ± 3 | 6.9 | 31 ± 25 | 42 |
aData for GS22-13 and GS22-15 are from Sargent et al. (19). GS22-13 and GS22-15 have tandem duplications identical to AK784 and GSAK1 except that they do not carry the 800 bp segment of non-homology or an I-SceI site in the second intron of APRT.
bData for AK784 and GSAK1 are from a single 12 pot fluctuation experiment analyzed to determine recombination rates, as described previously (23,24).