Table 3. I-SceI-stimulation of TK–APRT– colony formation in ERCC1+ and ERCC1– cell populations.
Cell line | DNA | TK–APRT– cells (×10–6)a | ||
---|---|---|---|---|
Experiment 1 | Experiment 2 | |||
AK784 | ERCC1+ | pCMV-β-galactosidase | <2 | 3.9 ± 3.8 |
AK784 | ERCC1+ | pCMV-I-SceI | 1100 ± 340 | 4100 ± 2600 |
GSAK1 | ERCC1– | pCMV-β-galactosidase | 11 ± 1.9 | 8.1 ± 4.3 |
GSAK1 | ERCC1– | pCMV-I-SceI | 62 ± 28 | 200 ± 130 |
aFor experiment 1 the frequencies of TK–APRT– cells are an average of results from four transfected wells. For experiment 2 the frequencies for pCMV-β-galactosidase transfections are an average of results from four transfected wells, whereas the frequencies for pCMV-I-SceI transfections are averaged from 20 transfected wells. Standard deviations are indicated.