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. 2024 May 7;15:3806. doi: 10.1038/s41467-024-47346-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Diagram of individual Stellate (Ste) and Su(Ste) repeat units. Su(Ste) repeats contain promoters at each end, allowing for bidirectional expression of complementary transcripts. Su(Ste) transcripts produced from the sense promoter are labeled as Su(Ste), and transcripts produced by the 1360TE/Hoppel TE are labeled as Su(Ste)-AS. b Widespread Su(Ste) antisense (Su(Ste)-AS, CR42418) transcript expression in testis. c Higher magnification of Su(Ste)-AS (CR41533) expression in gonialblast and spermatogonia nuclear foci and early spermatocyte cytoplasm. d Su(Ste) (CR42430) sense expression in spermatogonia nuclei and spermatocyte cytoplasm. e–g Expression patterns at the apical tip of the testis detected by HCR FISH using oligonucleotide probes that specifically target e Su(Ste)-AS (red), f Su(Ste) (blue) or g Ste (yellow). h–j Pairwise combinations of Su(Ste)-AS, Su(Ste) and Ste. k–m Higher magnifications of regions boxed in (h–j). Arrows indicate main nascent sites of expression. Blue arrowheads indicate minor sites of Su(Ste) localization that overlap with major sites of Ste localization. Scale bars = 20 μm.