Figure 3.

Incision of AF–, AAF– and AP–DNA adducts by UvrABC nuclease. (A) The 5′-terminally labeled DNA substrates (B0, 3 nM) containing an AF, AAF or AP adduct were incubated with UvrABC (10 nM UvrA, 250 nM UvrB and 100 nM UvrC) in UvrABC buffer at 37°C for the period indicated. The incision products were then analyzed on a 12% polyacrylamide sequencing gel. (B) Incision kinetics of the DNA substrates incised by UvrABC. Data represent the means ± SD of at least three independent experiments.