Fig. 3.

The MIRI with or without NAC treatment in second week of diabetic mice. A Expression of Gpx4 and Slc7a11 in mice with DM, assessed using Western blotting. B Levels of serum CK-MB, measured after reperfusion using the CK-MB ELISA kit. C and D Lipid peroxidation in DM mice, assessed by observing the changes in MDA and 15-F2t-IsoP levels. E Labile iron levels in DM mice, assessed using the Iron Colorimetric Assay Kit. F Alterations of GSH levels, assessed using the Glutathione Fluorometric Assay Kit. G Prussian blue stain showed abnormal iron (brown) deposition in myocardium. Iron infiltration was significantly increased after IRI in D2w mice, but there was no significant improvement after NAC treatment for 1 week. H Expression and the integrated optical density (IOD) of Ferritin assessed using IHC in myocardium (brown, arrowhead). Ferritin in D2w mice decreased significantly after IR, but it could still be detectable with IHC. Meanwhile, after NAC treatment, the ferritin levels were increased after ischemia by in D2w. The magnifications is 40 times. Data are expressed as mean ± SD, n = 8 mice per group. *p < 0.05, **p < 0.01, versus NC group; ^#p < 0.05, ^##p < 0.01, versus D2w group; ^p < 0.05, ^^p < 0.01, versus NC + IR group; ^$p < 0.05, ^$$p < 0.01, versus D2w + IR group. There is no significant statistical difference between groups without annotation symbols (p > 0.05). D2w Diabetes for 2 week, D2w + NAC Diabetes for 2 week and NAC treatment for 1 week