Fig. 6.

The effect of NAC on cardiac ultrastructure in diabetic mice with IR. A Typical morphological changes of ferroptosis in cardiomyocytes were observed using transmission electron microscopy. With the development of diabetes, ferroptosis-related injury during IR was gradually aggravated, which manifested as the increase and oxidation of lipid droplets in cardiac tissue, the disappearance of mitochondrial crest and the destruction of mitochondrial membrane increased. After 4 week of NAC treatment, mitochondrial damage was significantly alleviated. Erastin reverses the protective effect of NAC on cardiac ultrastructure. D1w Diabetes for a week, D2w Diabetes for 2 week, D5w Diabetes for 5 week, D2w + NAC Diabetes for 2 week and NAC treatment for 1 week, D5w + NAC Diabetes for 5 week with NAC treatment for 4 week