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HU induces Rfa1p phosphorylation. (A) Western blot analysis of Rfa1p from TWY397 cells harvested immediately before and after HU exposure. (B) Western blot analysis of Rfa1p from native extract of HU-treated TWY397 cells treated with λPPase and sodium orthovanadate as indicated. For both experiments, TWY397 cells were incubated at 30°C.
