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Radiation induces checkpoint-dependent Rfa1p phosphorylation. Wild-type and checkpoint mutant cells were untreated (–) or challenged with UV radiation (+; 60 J/m2). Rfa1p in denatured extracts of these cells was detected by western blot analysis. The strains employed were the same as those indicated in Figure 2A, except DLY285 (MATa mec1-1) was used instead of TWY308 (MATα mec1-1).
